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FAQs - Fluorescence Illumination

Many time-lapse live cell imaging experiments involve fluorescence illumination. Solent Scientific has always taken this need into account when designing new incubation chambers. Experiments are undertaken with little if no inconvenience.

Can I change the mercury lamp easily?

Yes. Access to the lamp house has to be easy. Some incubation chambers have extra access panels to help.

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Do I need to remove the incubation chamber to change the fluorescence cubes?

No. This is an everyday need of many laboratories so those chambers supplied to fluorescence microscopes accommodate this.

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Does the incubator generate problems during low light level observations?

No. Low light level measurements are actually improved by the use of a black incubation chamber.

Modern digital cameras are designed to observe low light signals across a wide wavelength range. Users of our inverted microscope chambers have reported significant improvements in their observations when using a black chamber. They have attributed this to a reduction in the infrared signal being seen by the camera.

Successful Multi-photon Microscopy involves optimising the signal to noise ratio of very small signals. To reduce the noise element laboratories are frequently totally blacked out. Even then the microscope’s detector can pick up even the light from a computer’s power switch LED. One user reports that after fitting a Solent Scientific black incubation chamber he can work in red light rather than total darkness.

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